Our results show that a similar phenotypic outcome results from increased inclusion of Sort1 exon 17b caused by abnormal TDP-43 function, leading to production of a soluble form of Sortilin that diverts trafficking of proBDNF away from the regulated secretory pathway, thereby impairing activity-dependent BDNF secretion. A disease-associated
Learn MoreBy changing HSPB1 and TDP-43's concentrations in vitro, the researchers found that the former ushered the latter into liquid droplets, but prevented the droplets from hardening into gels or solids. The chaperone also blocked TDP-43 from twisting into amyloid fibrils. In cells, most of the TDP-43-containing liquid droplets dissipated after the
Learn MoreTDP-43 deficiency leads to decreased dynactin 1 expression, blocking autophagic flux; it also reduces mTORC1 activity, leading to increased autophagy and lysosome gene expression via TFEB. Abnormal regulation of autophagy contributes to TDP-43-associated neurotoxicity. TDP-43 is required for raptor mRNA stability and thus for mTORC1 activity.
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Learn MoreIn normal cells, TDP-43 is mainly present in the nucleus and plays important roles in RNA regulation, such as transcriptional regulation,
Learn More1 As TDP-43 shuttles between nucleus and cytoplasm, it engages in diverse functions within both compartments (figure 1B). In the nucleus, TDP-43 regulates many
Learn MoreTDP-43 is abundantly expressed by all Sox10-positive Schwann cells (Figure 1A, Figure 1—figure supplement 1A, B).To elucidate the PNS-autonomous function of TDP-43, we specifically ablated TDP-43 from Schwann cells by combining the TDP-43 conditional allele (Tardbp fl/fl) (Chiang et al., ) with Dhh-Cre (Jaegle et al., 2003).In these conditional knockout (cKO) mice, TDP-43 expression is
Learn MoreThese results suggest that functions associated with RRM-1, such as (UG)n RNA binding, confer TDP-43 mobility in the nucleus. The function of RRM-2 remains
Learn More2016. 2. 1. · TDP-43 functions within a network of hnRNP proteins to inhibit the production of a truncated human SORT1 receptor Hum Mol Genet. Feb 1;25(3) :534-45. doi However, the pathological consequences of abnormal deposition of TDP-43 and other RNA-binding proteins remain unclear,
Learn MoreB) The TDP-43 protein is critical for mediating RNA metabolism. In the nucleus, TDP-43 is important for transcription and splicing of messenger RNA (mRNA), as well as maintaining RNA stability (pA) and transport to nucleus. In addition, TDP-43 regulates biogenesis of microRNA (miRNA) and processing of long non-coding RNA (lncRNA).
Learn More2019. 1. 29. · Abnormal TDP-43 function culminate in impaired secretion of neurotrophin BDNF, whose restoration is sufficient to rescue major disease phenotypes caused by aberrant TDP-43 activity. Knockdown, aggregation or disease-associated mutation of TDP-43 impair intracellular sorting and activity-dependent secretion of the neurotrophin brain-derived neurotrophic factor
Learn MoreThe two major RNA Binding Proteins involved in Amyotrophic Lateral Sclerosisi and Frontotemporal Dementia are TDP-43 and FUST/TLS. Both proteins are involved in regulating all aspects of RNA and RNA life cycle within neurons, from transcription, processing, and transport/stability to the formation of cytoplasmic and nuclear stress granules.
Learn MoreIn relation to its function, TDP-43 has a variety of diverse roles including gene transcription, RNA splicing, RNA shuttling and translation, and microRNA
Learn MoreTDP-43 binds both mRNA and DNA, thereby regulating mRNA splicing, stability and translation as well as gene transcription. Although early in vitro studies showed that TDP-43 preferentially bound RNAs via a GU dinucleotide repeat element [7], a clear consensus TDP-43 binding site in vivo has not been firmly established.
Learn Moreauto-feedback mechanisms, which involve TDP-43 binding to its own 3' untranslated region [15,22]. Overexpression of TDP-43 leads to down-regulation of the endogenous TDP-43 [23,24], and blocking expression of one allele leads to a compensatory increase in the expression of the other allele [25-27]. The tight regulation of TDP-43 levels is
Learn MoreAlterations in TDP-43 are commonly found in patients suffering from amyotrophic lateral sclerosis (ALS) and the genetic suppression of the conserved homologue in Drosophila (TBPH) provokes alterations in the functional organization of motoneuron
Learn MoreTDP-43 also acts as the transcriptional repressor and/or insulation regulator for the spatiotemporal regulation of the ACRV1 (SP-10) gene [140, 141]. TDP-43
Learn MoreTDP-43 is an evolutionarily conserved ubiquitously expressed DNA/RNA-binding protein. Although recent studies have shown its association with a variety of neurodegenerative disorders, the function of TDP-43 remains poorly understood. Here we address TDP-43 function using spermatogenesis as a model system. We previously showed that TDP-43 binds to the testis-specific mouse acrv1 gene promoter
Learn MoreCritically, TDP-43 autoregulation is perturbed, leading to a gain of TDP-43 function and altered splicing of Mapt, another pivotal dementia-associated gene. Furthermore, a new approach to stratify transcriptomic data by phenotype in differentially affected mutant mice revealed 471 changes linked with improved behavior.
Learn More2021. 4. 23. · TDP-43 aggregation and redistribution have been recognised as a hallmark of amyotrophic lateral sclerosis, frontotemporal dementia and other neurological disorders. While TDP-43 has been studied extensively in neuronal tissues, TDP-43 inclusions have also been described in the muscle of inclusion body myositis patients, highlighting the need to
Learn MoreTDP-43 has diverse cellular roles in regulating RNA splicing and RNA stability as well as other gene regulatory functions 3, 4, 5. High-throughput sequencing approaches have shown that TDP-43 binds
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